Medicinal potential of antimicrobial peptides from two plants against Bacillus cereus and Staphylococcus aureus

Submitted: 24 November 2023
Accepted: 29 December 2023
Published: 1 February 2024
Abstract Views: 365
PDF: 194
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Bacillus cereus and Staphylococcus aureus are the most important bacteria that cause nosocomial infection and are resistant to antibiotics. Crude proteins from Cassia fistula and Ricinus communis were isolated to study their medicinal potential against Bacillus cereus, and Staphylococcus aureus. Extraction of the crude proteins from plants was done by phosphate buffer saline (PBS) and Tris NaCl buffer by using the roots and seeds of both plants. Antimicrobial activity was checked against bacterial strains by using agar disc diffusion and agar well diffusion methods. Zones of inhibitions were measured. On well diffusion method, PBS buffer protein extract of C. fistula roots showed a maximum zone of inhibition of 25 mm against B. cereus. Tris NaCl buffer extracts of C. fistula roots and seeds showed zones of inhibition of 12mm and 5mm respectively against S. aureus while Ricinus communis roots showed a zone of 12mm against B. cereus. Because the protein of the plants showed good antimicrobial activity, we can use these plants against various diseases caused by Bacillus cereus and Staphylococcus aureus.

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Ahmad F, Anwar F, Hira S. Review on medicinal importance of Fabaceae family. Pharmacologyonline 2016;3:151-7.
Sen T, Samanta SK. Medicinal plants, human health and biodiversity: a broad review. Adv Biochem Eng Biotechnol 2015;147:59-110. DOI: https://doi.org/10.1007/10_2014_273
Chandra H, Bishnoi P, Yadav A, et al. Antimicrobial resistance and the alternative resources with special emphasis on plant-based antimicrobials—a review. Plants 2017;6:16. DOI: https://doi.org/10.3390/plants6020016
De-Paula V, Razzera G, Medeiros L, et al. Evolutionary relationship between defensins in the Poaceae family strengthened by the characterization of new sugarcane defensins. Plant Mol Biol 2008;68:321-35. DOI: https://doi.org/10.1007/s11103-008-9372-y
Doughari J. An overview of plant immunity. J Plant Pathol Microbiol 2015;6:1-11. DOI: https://doi.org/10.4172/2157-7471.1000322
Souza TP, Dias RO, Silva-Filho MC. Defense-related proteins involved in sugarcane responses to biotic stress. Genet Mol Biol 2017;40;360-72. DOI: https://doi.org/10.1590/1678-4685-gmb-2016-0057
van Loon LC, Rep M, Pieterse CM. Significance of inducible defense-related proteins in infected plants. Annu Rev Phytopathol 2006;44:135-62.
Freeman B, Beattie G. An Overview of Plant Defenses against Pathogens and Herbivores. The Plant Health Instructor. 2008. DOI: https://doi.org/10.1094/PHI-I-2008-0226-01
van Loon LC, Rep M, Pieterse CM. Significance of inducible defense-related proteins in infected plants. Annu Rev Phytopathol 2006;44;135-62. DOI: https://doi.org/10.1146/annurev.phyto.44.070505.143425
Noonan J, Williams WP, Shan X. Investigation of Antimicrobial Peptide Genes Associated with Fungus and Insect Resistance in Maize. Int J Mol Sci, 2017;18:1938. DOI: https://doi.org/10.3390/ijms18091938
Thomma BP, Cammue BP, Thevissen K. Plant defensins. Planta 2002;216:193-202. DOI: https://doi.org/10.1007/s00425-002-0902-6
Al Akeel R, Al-Sheikh Y, Mateen A, et al. Evaluation of antibacterial activity of crude protein extracts from seeds of six different medical plants against standard bacterial strains. Saudi J Biol Sci 2014;21:147-51. DOI: https://doi.org/10.1016/j.sjbs.2013.09.003
Altaher AA. Preparation of Poly-Anhydride from Azelaic Acid. Sudan University of Science and Technology (SUST) 2014.
Wink M, Alfermann AW, Franke R, et al. Sustainable bioproduction of phytochemicals by plant in vitro cultures: anticancer agents. Plant Genetic Resources 2005;3:90-100. DOI: https://doi.org/10.1079/PGR200575
Mary Kensa V, Syhed Yasmin S. Phytochemical screening and antimicrobial activity on ricinus communisl. Plant Sciences Feed 2011;1:167-73.
Jeyaseelan EC, Jashothan PJ. In vitro control of Staphylococcus aureus (NCTC 6571) and Escherichia coli (ATCC 25922) by Ricinus communis L. Asian Pac J Trop Biomed 2012;2:717-21. DOI: https://doi.org/10.1016/S2221-1691(12)60216-0
Chauhan N, Bairwa R, Sharma K, Chauhan, N. Review on Cassia fistula. Int J Res Ayurveda Pharm 2011;2:426-30.
Pawar AV, Killedar SG. Uses of Cassia fistula Linn as a medicinal plant. Int J Adv Res Dev 2017;2.
Singh S, Singh SK, Yadav A. A review on Cassia species: Pharmacological, traditional and medicinal aspects in various countries. Ame J Phytomed Clin Therap 2013;1:291-312.
Bhalerao SA, Verma DR, Teli N, et al. Bioactive constituents, ethnobotany and pharmacological prospectives of cassia tora linn. Int J Bioassays 2013;2:1421-7.
Kumar VP, Chauhan NS, Padh H, Rajani M. Search for antibacterial and antifungal agents from selected Indian medicinal plants. J Ethnopharmacol 2006;107:182-8. DOI: https://doi.org/10.1016/j.jep.2006.03.013
Jafari-Sales A, Jafari B, Khaneshpour H, Pashazadeh M. Antibacterial effect of methanolic extract of rosa damascena on standard bacteria Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa in vitro. Int J Nat Life Sci 2020;4:40-6.
Boukhatem MN, Ferhat MA, Kameli A, Saidi F, Kebir HT. Lemon grass (Cymbopogon citratus) essential oil as a potent anti inflammatory and antifungal drugs. Libyan J Med 2014;9:25431. DOI: https://doi.org/10.3402/ljm.v9.25431
Montesinos E. Antimicrobial peptides and plant disease control. FEMS microbiology letters 2007;270:1-11. DOI: https://doi.org/10.1111/j.1574-6968.2007.00683.x
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54. DOI: https://doi.org/10.1006/abio.1976.9999
Naqvi SAR, Shah SMA, Kanwal L, et al. Antimicrobial and antihypercholesterolemic activities of pulicaria gnaphalodes. Dose Response 2020;18. DOI: https://doi.org/10.1177/1559325820904858
Jahangirian H, Haron MJ, Shah MH, et al. Well diffusion method for evaluation of antibacterial activity of copper phenyl fatty hydroxamate synthesized from canola and palm kernel oils. Dig J Nanomater Biostructures 2013;8;1263-70.
Nagel TE, Chan BK, De Vos D, et al. The developing world urgently needs phages to combat pathogenic bacteria. Front Microbiol 2016;7:882. DOI: https://doi.org/10.3389/fmicb.2016.00882
Shakya AK. Medicinal plants: future source of new drugs. Int J Herb Med 2016;4:59-64.
Al-Mamun MA, Akter Z, Uddin MJ, et al. Characterization and evaluation of antibacterial and antiproliferative activities of crude protein extracts isolated from the seed of Ricinus communis in Bangladesh. BMC Complement Altern Med 2016;16:211. DOI: https://doi.org/10.1186/s12906-016-1185-y
Verma SK, Yousuf SAJAD, Singh SK, et al. Antimicrobial potential of roots of Riccinus communis against pathogenic microorganisms. Int J Pharm Bio Sci 2011;2:545-8.
Petnual P, Sangvanich P, Karnchanatat A. A lectin from the rhizomes of turmeric (Curcuma longa L.) and its antifungal, antibacterial, and α-glucosidase inhibitory activities. Food Sci Biotechnol 2010;19:907-16. DOI: https://doi.org/10.1007/s10068-010-0128-5
Małajowicz J, Kuśmirek S. Structure and properties of ricin–the toxic protein of Ricinus communis. Postepy biochemii 2019;65;103-8. DOI: https://doi.org/10.18388/pb.2019_249
Nayan RB, Shukla VJ, Acharya RN, Rajani DP. Antimicrobial screening of seed extracts of Cassia fistula linn. Int J Adv Pharma Nanotechnol 2011;3:1-8.
Channabasappa HS, Shrinivas JD, Venkatrao KH. Evaluation of antibacterial and Antitubercular activity of Cassia fistula Linn root. Int J Res Pharm Sci 2015;6:82-4.
Yadava RN, Verma V. A new biologically active flavone glycoside from the seeds of Cassia fistula (Linn.). J Asian Nat Prod Res 2003;5:57-61. DOI: https://doi.org/10.1080/1028602031000080478
Awal M, Ahsan S, Haque E, et al. In-vitro antibacterial activity of leaf and root extract of Cassia fistula. Dinajpur Medical College 2010;3:10-3.
Jabeen R, Jamil A, Shahid M, Ashraf, M. Bioactive Potential of 1ormal and Fungal Stressed Extracts from Ricinus Communis L. (Castor) Whole Plant Relative Potential of Protein/PeStide. Oxid Commun 2015;38:1612-21.
Habiba U, Nisar J, Choohan MA, et al. Antibacterial Activity of Tris NaCl and PBS Buffer Protein Extract of Cassia fistula, Saccharum officinarum, Albizia lebbeck and Cymbopogon citrates Against Bacterial Strains. Dose-Response 2021;19. DOI: https://doi.org/10.1177/1559325821992239
Amjad K, Aamir MN, Choohan MA, et al. Preparation and characterization of magnetic nanoparticles loaded with antimicrobial agent. J Contemp Pharm 2021;5:53-6. DOI: https://doi.org/10.56770/jcp2021522

How to Cite

Jabeen, R., Javed, E., Habiba, U., Choohan, M. A., Asim, M., Alatawi, F. S., Ishfaq , H., & Nisar, J. (2024). Medicinal potential of antimicrobial peptides from two plants against <i>Bacillus cereus</i> and <i>Staphylococcus aureus</i>. Italian Journal of Medicine, 18(1). https://doi.org/10.4081/itjm.2024.1670