https://doi.org/10.4081/itjm.2023.1607
Urotensin II promotes the proliferation and secretion of vascular endothelial growth factor in rat dermal papilla cells by activating the Wnt-β-catenin signaling pathway
Submitted: 1 June 2023
Accepted: 23 June 2023
Published: 27 June 2023
Accepted: 23 June 2023
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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Introduction. Urotensin II (U II) is a kind of active peptide with a variety of biological effects, such as promoting cell proliferation and endocrine effects. The aim of this study is to investigate the effect of urotensin II on the proliferation and secretion of vascular endothelial growth factor (VEGF) in cultured rat dermal papilla cells (DPCs), and to explore its molecular mechanism. Materials and Methods. We used the DPCs isolated from the thoracic aortas of Wistar-Kyoto rats to run the CCK8 and ELISA assay, RC-PCR and Western blotting techniques to identify the effect of Urotensin II on the proliferation and secretion of VEGF in DPCs, data were analyzed by one-way ANOVA or t-test. Results. U II can increase the mRNA expression of proliferation markers Ki67 and PCNA. In addition, the Wnt/β-catenin pathway was activated by U II, but Wnt inhibitor DKK1 reversed the effect of U II. Conclusions. U II promoted the proliferation and secretion of VEGF in rat DPCs through activation of the Wnt-β-catenin signaling pathway.
Xiong Y, Liu Y, Song Z, et al. Identification of Wnt/beta-catenin signaling pathway in dermal papilla cells of human scalp hair follicles: TCF4 regulates the proliferation and secretory activity of dermal papilla cell. J Dermatol 2014;41:84-91.
DOI: https://doi.org/10.1111/1346-8138.12313
Zerbinati N, Sommatis S, Maccario C, et al. In Vitro hair growth promoting effect of a noncrosslinked hyaluronic acid in human dermal papilla cells. Biomed Res Int 2021;2021:5598110.
DOI: https://doi.org/10.1155/2021/5598110
Yano K, Brown LF, Detmar M. Control of hair growth and follicle size by VEGF-mediated angiogenesis. J Clin Invest 2001;107:409-17.
DOI: https://doi.org/10.1172/JCI11317
Liu X, Ji T, Hu H, et al. The hair growth-promoting effect of gardenia florida fruit extract and its molecular regulation. Evid Based Complement Alternat Med 2022;2022:8498974.
DOI: https://doi.org/10.1155/2022/8498974
Liu J, Xiao Q, Xiao J, et al. Wnt/beta-catenin signalling: function, biological mechanisms, and therapeutic opportunities. Signal Transduct Target Ther 2022;7:3.
DOI: https://doi.org/10.1038/s41392-021-00762-6
Choi BY. Targeting Wnt/beta-catenin pathway for developing therapies for hair Loss. Int J Mol Sci 2020;21:4915.
DOI: https://doi.org/10.3390/ijms21144915
Kishimoto J, Burgeson RE, Morgan BA. Wnt signaling maintains the hair-inducing activity of the dermal papilla. Genes Dev 2000;14:1181-5.
DOI: https://doi.org/10.1101/gad.14.10.1181
Svistunov AA, Tarasov VV, Shakhmardanova SA, et al. Urotensin II: molecular mechanisms of biological activity. Curr Protein Pept Sci 2018;19:924-34.
DOI: https://doi.org/10.2174/1389203718666170829162335
Watanabe T, Pakala R, Katagiri T, Benedict CR. Synergistic effect of urotensin II with mildly oxidized LDL on DNA synthesis in vascular smooth muscle cells. Circulation 2001;104:16-8.
DOI: https://doi.org/10.1161/hc2601.092848
Soni H, Adebiyi A. Urotensin II-induced store-operated Ca(2+) entry contributes to glomerular mesangial cell proliferation and extracellular matrix protein production under high glucose conditions. Sci Rep 2017;7:18049.
DOI: https://doi.org/10.1038/s41598-017-18143-x
Yu X, Wang P, Shi Z, et al. Urotensin-II-mediated reactive oxygen species generation via NADPH oxidase pathway contributes to hepatic oval cell proliferation. PLoS One 2015;10:e0144433.
DOI: https://doi.org/10.1371/journal.pone.0144433
Khan K, Albanese I, Yu B, et al. Urotensin II, urotensin-related peptide, and their receptor in aortic valve stenosis. J Thorac Cardiovasc Surg 2019;161:e1-e15.
DOI: https://doi.org/10.1016/j.jtcvs.2019.09.029
Pereira-Castro J, Bras-Silva C, Fontes-Sousa AP. Novel insights into the role of urotensin II in cardiovascular disease. Drug Discov Today 2019;24:2170-80.
DOI: https://doi.org/10.1016/j.drudis.2019.08.005
Han L, Liu B, Chen X, et al. Activation of Wnt/beta-catenin signaling is involved in hair growth-promoting effect of 655-nm red light and LED in vitro culture model. Lasers Med Sci 2018;33:637-45.
DOI: https://doi.org/10.1007/s10103-018-2455-3
Xiao S, Deng Y, Mo X, et al. Promotion of hair growth by conditioned medium from extracellular matrix/stromal vascular fraction gel in C57BL/6 mice. Stem Cells Int 2020;2020:9054514.
DOI: https://doi.org/10.1155/2020/9054514
Sun X, Kaufman PD. Ki-67: more than a proliferation marker. Chromosoma 2018;127:175-86.
DOI: https://doi.org/10.1007/s00412-018-0659-8
Gonzalez-Magana A, Blanco FJ. Human PCNA structure, function and interactions. Biomolecules 2020;10:570.
DOI: https://doi.org/10.3390/biom10040570
Yu Q, Wei P, Xu L, et al. Urotensin II enhances advanced aortic atherosclerosis formation and delays plaque regression in hyperlipidemic rabbits. Int J Mol Sci 2023;24:3819.
DOI: https://doi.org/10.3390/ijms24043819
Albertin G, Guidolin D, Sorato E, et al. Urotensin-II-stimulated expression of pro-angiogenic factors in human vascular endothelial cells. Regul Pept 2011;172:16-22.
DOI: https://doi.org/10.1016/j.regpep.2011.08.001
Ahmed MI, Alam M, Emelianov VU, et al. MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway. J Cell Biol 2014;207:549-67.
DOI: https://doi.org/10.1083/jcb.201404001
Millar SE. Molecular mechanisms regulating hair follicle development. J Invest Dermatol 2002;118:216-25.
DOI: https://doi.org/10.1046/j.0022-202x.2001.01670.x
Zhang Y, Tomann P, Andl T, et al. Reciprocal requirements for EDA/EDAR/NF-kappaB and Wnt/beta-catenin signaling pathways in hair follicle induction. Dev Cell 2009;17:49-61.
DOI: https://doi.org/10.1016/j.devcel.2009.05.011
Andl T, Reddy ST, Gaddapara T, Millar SE. WNT signals are required for the initiation of hair follicle development. Dev Cell 2002;2:643-53.
DOI: https://doi.org/10.1016/S1534-5807(02)00167-3
Huelsken J, Vogel R, Erdmann B, et al. Beta-catenin controls hair follicle morphogenesis and stem cell differentiation in the skin. Cell 2001;105:533-45.
DOI: https://doi.org/10.1016/S0092-8674(01)00336-1
Narhi K, Jarvinen E, Birchmeier W, et al. Sustained epithelial beta-catenin activity induces precocious hair development but disrupts hair follicle down-growth and hair shaft formation. Development 2008;135:1019-28.
DOI: https://doi.org/10.1242/dev.016550
Zhang Y, Andl T, Yang SH, et al. Activation of beta-catenin signaling programs embryonic epidermis to hair follicle fate. Development 2008;135:2161-72.
DOI: https://doi.org/10.1242/dev.017459
Supporting Agencies
This work was supported by the Special Fund for Economic and Scientific Development in Longgang District, Shenzhen City, Guangdong Province (No. LGWJ2022-32).How to Cite
Liao, C., Huang, Z., Chen, L., Fan, X., Peng, J., Tan, X., Yang, J., & Zhang, X. (2023). Urotensin II promotes the proliferation and secretion of vascular endothelial growth factor in rat dermal papilla cells by activating the Wnt-β-catenin signaling pathway. Italian Journal of Medicine, 17(1). https://doi.org/10.4081/itjm.2023.1607
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